How can deinococcus radiodurans be used in bioremediation

This may take some time to load. Loading related content. Jump to main content. Jump to site search. You do not have JavaScript enabled. Please enable JavaScript to access the full features of the site or access our non-JavaScript page. Issue 28, From the journal: Chemical Communications. You have access to this article. Please wait while we load your content Something went wrong. Try again? Cited by.

Download options Please wait Supplementary information PDF K. Article type Communication. This may ultimately result not only in a more complete understanding of the radiation resistance of this bacterium, but also to the discovery of novel DNA repair systems, applicable to an understanding of the mechanisms of higher organisms such as man. Analysis of paralogs in Deinococcus has revealed several unique protein families. In addition, specific expansions of several other families including phosphatases, proteases, acyltransferases, and Nudix family pyrophosphohydrolases were detected.

Genes that potentially affect DNA repair and stress responses and recombination were investigated in this article. These observations suggest that several different biological mechanisms contribute to the multiple DNA repair-dependent phenotypes of this organism. Then D. This analysis is a single substantiation of function of Nudix protein family with is taken from D. Article :. DOI: Need Help? Bioremediation by Use of Genetic Engineering There have been several studies where genes of pollutant degrading bacteria, such as Pseudomonas have been added to this highly radiation resistant bacteria.

While D. Scientists transferred a set of genes from pseudomonas into D. This was accomplished by fusing a gene encoding toluene dioxygenase, which is an enzyme that degrades toluene, to a Deinococcus radiodurans promoter a site that activates the gene.

Then the DNA was inserted into one of the bacterium's chromosomes, which utimately resulted in a recombinant bacterium. The end result gives you a bacterium that is capable of degrading toluene and other organic compounds in a high-radiation environment.

This genetically engineered bacterium can withstand toluene and trichloroethylene at levels that exceeds the levels found at many radioactive waste sites. Even after the D.